Tag

Part:BBa_K1486028

Designed by: EPFL iGem team 2014   Group: iGEM14_EPF_Lausanne   (2014-09-27)

Yeast optimized sfGFP N-terminus (1-214)

This part is the N-terminus of a Yeast optimized sfGFP. It contains the 214 first amino-acids, and needs to bind the 24 amino acids long C-terminus fragment to recover fluorescence. Its goal is to provide readout in protein complementation assays, where two proteins tagged with the two termini come in conctact.

Source

It was extracted by PCR from the plasmid we ordered to addgene:44901 pFA6a-link-yoSuperfolderGFP-Kan.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/gfp
//chassis/eukaryote/yeast
//direction/forward
//function/reporter/fluorescence
Parameters
biology
chassisSaccharomyces Cerevisiae
colorGreen
directionForward
emission510
excitation480
functionReporter